Immune changes at the injured sites in oxazolone-induced ulcerous colitis: Influence of ozone therapy

Impaired immunoregulation and development of autoimmune response to antigens own intestinal microbiota inflammation-altered colonic cells represent the key links in pathogenesis inflammatory bowel diseases. Multimodal biological effects ozone presunme usage local systemic therapy complex treatment many diseases gastrointestinal tract. The aim our work was study intraperitoneal rectal upon immune parameters lesion focus oxazolone-induced ulcerative colitis course time. carried out on 64 adult male inbred Wistar rats weighing 240±20 g. Experimental produced by oxazolone (4-ethoxymethylene-2-phenyl-2-oxazolin-5-one) (SigmaAldrich, USA). ozone-oxygen mixture injected intraperitoneally or rectally at a concentration 1.0-1.2 mg/l, once day, volume 10 ml, 6-day course. results experiments were recorded days +2, +4 +6. concentrations IL-17 IL-23 determined homogenate tissues (Bender Medsystems, Austria) using Personal LAB analyzer; expression CD4 FoxP3 lymphocytes immunohistochemistry technique (ElisaKit, China). observed tissue damage large intestine showed an increase from day 2 6 colitis. total number significantly increased experimental colitis, with parallel decrease CD4+ FoxP3-positive T lymphocytes. severity changes focus. Intraperitoneal administration associated significant reduction lymphocyte contents damaged 6th whereas numbers positive normalized day. levels 6, lower values as compared non-treated animals. Rectal ACS led normalization intact proinflammatory cytokines (IL-17 IL-23) decreased group animals without treatment, which could be due anti-inflammatory properties ozone.